A rapid and reliable detection procedure of Atlantic trout introgression at the diagnostic lactate dehydrogenase chain‐1 gene

The Italian-native Mediterranean brown trout (Salmo ghigii) is a seriously threatened freshwater fish, especially by anthropogenic hybridisation with the domestic strains of Atlantic origin that have been repeatedly released into wild for angling. A PCR-restriction fragment length polymorphism (RFLP) assay diagnostic lactate dehydrogenase chain-1 (LDH-C1) gene sequences has routinely applied to distinguish exotic from native lineages and detect introgression signals in populations. Here, we used dermal swab DNA obtained 28 improve laboratory procedures genetically characterise samples at LDH-C1gene through (1) capillary electrophoresis analysis RFLP fragments (2) optimisation single nucleotide analysable mini-sequencing approaches. developed methods were fully consistent those traditional approach, but their analytical process almost entirely automated digitalised, thus improving result readability accuracy detection alien introgressed traces